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In the vast galaxy of life science research, proteomics serves as a crucial tool for exploring the mysteries of life, continually pushing the boundaries of technology. Today, the emergence of 4D proteomics has brought about a revolutionary leap in this field. It not only redefines the limits of proteomics analysis but also leads the future direction of research with its unique advantages.
In traditional proteomics research, we rely on three main dimensions for protein separation and identification: retention time, mass-to-charge ratio, and ion intensity. However, as scientific research deepens, these dimensions are no longer sufficient to meet the increasingly complex and precise analytical needs. 4D proteomics ingeniously introduces a fourth dimension—ion mobility—achieving a comprehensive and in-depth analysis of protein samples. This innovation significantly enhances scanning speed and detection sensitivity, upgrading the identification depth, detection cycle, and quantitative accuracy in proteomics.
In the face of the complexity of proteomic samples and the limitations of traditional mass spectrometers in collection speed and sensitivity, improving analysis throughput, quantitative stability, and data integrity has become an urgent issue for researchers. Although the traditional Data-Dependent Acquisition (DDA) strategy is powerful, its random target selection can lead to missing value problems. On the other hand, the Data-Independent Acquisition (DIA) strategy retrieves all ion fragment information without omission, but its data complexity and quantitative reliability pose new challenges.
Against this backdrop, 4D-DIA proteomics, based on diaPASEF technology, emerged. It cleverly integrates the advantages of 4D proteomics and DIA technology, retaining the comprehensiveness of DIA services while using the ion mobility separation of the 4D platform to reduce spectral complexity and enhance quantitative reliability. This innovative "dual-sword combination" undoubtedly opens up new avenues for proteomics research.
Experimental data is the best proof of a technology's strength. In a 120-minute single injection experiment using 200ng HeLa lysate, 4D-DIA technology demonstrated its astonishing analytical capabilities—identifying over 7,500 proteins, with 6,974 proteins consistently quantified across three experiments, achieving a data integrity of 96%. In comparison, traditional DIA technology typically requires microgram-level samples to reach similar levels. Remarkably, even with only 10ng of sample, 4D-DIA could still identify 3,323 proteins and maintain 85% data integrity, challenging its sensitivity limits once again successfully.
As 4D proteomics technology continues to mature and improve, its application prospects in various fields of life sciences will expand. Whether it's systematic screening of large cohort samples, in-depth exploration of complex disease mechanisms, or precise targeting for clinical diagnosis and treatment, 4D proteomics will play an irreplaceable role. As one of leading Omics services supplier, INOMIXO keep pace with this technology, and believe it lead proteomics research into a new era, making more remarkable contributions to revealing the mysteries of life and promoting human health.
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