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Targeted lipidomics involves a focused analysis of a predetermined set of lipids. Using high-resolution mass spectrometry and isotope-labeled standards, our targeted analysis technique employs parallel reaction monitoring to specifically capture signals of target lipid molecules. This allows for the absolute quantification of these lipids, fulfilling the requirements for targeted detection and validation of lipid classes.


Targeted Lipidomics Advantages


  • Wide Applicability: No species restrictions, no standard limitations.

  • High Throughput: Simultaneous detection of dozens of lipid molecules, saving on sample volume and experimental costs.

  • Quantification Ability: Sensitivity up to parts per million (ppm) level, with a linear range spanning 5-6 orders of magnitude.


Targeted Lipidomics Technical Roadmap


Targeted Lipidomics Technical Roadmap


Targeted Lipidomics Sample Requirements

Sample type

sample

Recommended

Biological duplication

Body fluid

Seminal plasma. serum, urine, saliva, sperm, secretions……

≥200 μL/sample

Clinical ≥30 samples/group

Animal ≥10 samples /group

Plant ≥6 samples /group

Cell ≥6 samples /group

Animal tissue

Cancer and adjacent non-tumor, brain, liver tissues……

≥200 mg/sample

Plant

Roots, stems, leaves

≥200 mg/sample

Stool

Feces, intestinal contents

≥200 mg/sample

Cells

Suspension and adherent cells

≥107 /sample

Bacteria

Microbes (bacterial precipitate)

≥200 mg/sample

Others

others

Customized



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