

Targeted metabolomics relies on LC-MS/MS mass spectrometry to detect targeted metabolites. By referencing their standards and refining the acquisition process, this method prioritizes specificity, sensitivity, and accuracy, enabling both relative and absolute quantification of targeted metabolites.

Targeted Metabolomics Testing Category

Category	Detection
Energy metabolism	Glycolysis, TCA, oxidative phosphorylation, and various other glucose metabolic pathways involve nearly 40 metabolites related to glucose metabolism.
Amino acid	Common 30 kinds of amino acids and their derivatives.
Bile acids	Nearly 40 kinds of free and conjugated bile acids.
Fatty acids	Including more than 30 kinds of C6~C20 medium and long-chain fatty acids, and C2~C6 short-chain fatty acids.
Neurotransmitters	Acetylcholine, catecholamines, serotonin, GABA, and 33 kinds of neurotransmitters.
Arachidonic acid	Covering classical metabolic pathways such as COX and LOX.
Tryptophan metabolic pathway	Tryptophan, indole-3-acetic acid, indole, serotonin, melatonin, and over 30 kinds of other compounds.
Vitamins	Water-soluble and fat-soluble vitamins
TMAO	Choline, betaine, TMA (trimethylamine), TMAO (trimethylamine N-oxide), etc.
Plant hormones	Common 20+ types of plant hormones.
Flavonoids	Flavone, flavonoid glycosides, isoflavones, and 40+ similar compounds.

Targeted Metabolomics Workflow

Targeted Metabolomics Sample Requirements

Sample type	sample	Recommended
Body fluid	Seminal plasma. serum, urine, saliva, sperm, secretions……	≥200 μL/sample
Animal tissue	Cancer and adjacent non-tumor, brain, liver tissues……	≥200 mg/sample
Plant	Roots, stems, leaves	≥200 mg/sample
Plant	Nectar, * root exudates	≥200 μL/sample
Stool	Feces, intestinal contents	≥200 mg/sample
Cells	Suspension and adherent cells	≥10⁷ /sample
Culture medium	* Cell supernatant, * Fermentation broth	≥200 μL/sample
Bacteria	Microbes (bacterial precipitate)	≥200 mg/sample
Others	Environmental samples or others	Customized