Name: PTMs Proteomics
Brand: INOMIXO.

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Many crucial biological processes in organisms are not only regulated by the relative abundance of proteins but also by various temporally and spatially specific, reversible post-translational modifications (PTMs). Therefore, elucidating the occurrence patterns of PTMs is essential for unraveling the diverse and complex biological functions of proteins. Currently, over 400 different types of PTMs have been identified, each exerting various effects on cellular functions such as protein stability, metabolism, and signal transduction. These modifications include phosphorylation, glycosylation, methylation, acetylation, amidation etc. INOMIXO offers post-translational modification prediction services to assist in this area.

Common Types of PTMS Proteomics

Modifications	Function	Technology
Phosphorylation (S/T/Y)	Signal Transduction	DIA modification TMT modification 4D-label free modification
Ubiquitination (K)	Protein Expression Regulation	4D-label free modification
Acetylation (K)	Metabolic Regulation	TMT modification 4D-label free modification
N-Glycosylation (D)	Alteration of protein conformation	label free modification
O-GlcNAcylation (S/T)	Nutrient and stress sensors	4D-label free modification
Others (Methylation, succinylation, acetylation, propionylation, butyrylation)		4D-label free modification

DIA modification: Based on DIA holographic scanning technology, unbiased collection of all signals is conducted, making it suitable for ultra-high depth, stable, accurate, and large-cohort samples analysis.
TMT modification: Based on labeled fractionation technology, it is suitable for analyzing modifications with high depth and high parallelism within 16 samples.
4D-label free modification: Based on ion digestion separation, it reduces sample consumption, making it suitable for trace-level, cost-effective analysis.

Sample Requirements of PTMS Proteomics

Sample	Phos				Ac		Ub/Me	N-Glyc		O- Glyc
Sample	DIA	TMT	4D-label free	TMT		4D-label free			Label free		4D-label free
Animal tissue	75mg~2g	50mg~2g	30mg~1g	75mg~2g		75mg~2g			30mg~1g		75mg~2g
Mollusca	70mg~2g	50mg~2g	30mg~1g	75mg~2g		70mg~2g			30mg~1g		100mg~2g
Soft plant tissue	250mg~2g	200mg~2g	100mg~1g	300mg~2g		250mg~2g			100mg~1g		500mg~2g
Fruit and Seed	1g~5g	1g~5g	0.5g~2g	1g~5g		1g~5g			0.5g~2g		1g~5g
Cells	3~10×10⁷	2~10×10⁷	1.5~5×10⁷	2~10×10⁷		3~10×10⁷			1.5~5×10⁷		1~10×10⁷
Bacteria	120mg~2g	100mg~2g	50mg~2g	150mg~2g		120mg~2g			50mg~2g		200mg~2g