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The TMT  labeling proteomics technology is a relative and absolute quantification technique for peptide-based proteomics. It utilizes 6, 10, or 16 isotopic labels to specifically label the amino groups of peptides. Through tmt tandem mass tag spectrometry analysis, it enables the simultaneous comparison of protein expression levels across up to 16 different samples. TMT  labeling is a high-throughput screening technique widely used in quantitative proteomics research.

TMT Labeling Proteomics Workflow

TMT Based Quantitative Proteomics


  • Uniform processing of samples labeled in the same batch reduces sample handling and experimental errors, resulting in high experimental reproducibility.

  • High quantitative stability is achieved when samples are labeled in the same batch.

  • The use of fractionation methods significantly increases the depth of protein identification.



TMT Labeling  Proteomics Sample Requirements

Sample type

Sample

Recommended Volume

Animal tissue

Brain, heart, liver, spleen, lungs, kidneys, muscles, skin, or other tissues

≥30 mg

Plant tissue

Soft tissues of woody and herbaceous plants

≥100 mg

Body fluid

Plasma. serum

≥100 μL

Cells

Suspension or adherent cells

≥1×107

Cell culture supernatant

≥5 mL

Exosomes

Isolated exosomes

≥100 μg

* Please contact info@inomixo.com if you have any questions regarding the samples.


TMT Proteomics Provides Distinct Advantages Over iTRAQ

Quantitative Accuracy:TMT Offers higher quantitative accuracy and precision due to improved isotopic purity and reduced interference.

Multiplexing Capability:TMT Supports higher multiplexing (up to 16-plex), allowing simultaneous analysis of more samples.

Sensitivity:TMT Provides better sensitivity and dynamic range, ideal for detecting low-abundance proteins.

Data Quality:TMT Produces cleaner spectra with less background noise, leading to more reliable data.

Adoption and Support: TMT Widely adopted with extensive support and optimized protocols available.


Why is INOMIXO's TMT Proteomics Service the Better Choice?

Advanced Technology: INOMIXO utilizes state-of-the-art TMT labeling techniques, ensuring high precision and accuracy in protein quantification.

High Multiplexing Capability: Our service supports up to 16-plex TMT, allowing for the simultaneous analysis of multiple samples, which enhances throughput and efficiency.

Expertise and Experience: INOMIXO's team comprises seasoned professionals with extensive experience in proteomics, ensuring reliable and high-quality results.

Comprehensive Data Analysis: We provide detailed and comprehensive data analysis, including statistical validation and bioinformatics support, to facilitate meaningful insights.

Customer Support: INOMIXO offers exceptional customer CRO services, including personalized consultation and ongoing support throughout your project.


Get In Touch With INOMIXO
Get In Touch With INOMIXO
Connect with INOMIXO today to unlock the potential of biomedical research and biopharmaceutical analysis.