Biomarker Quantification by LC/MS

INOMIXO Target Protein Quantification Service

At INOMIXO, our specialized service primarily offers protein quantification utilizing LC/MS to identify and quantify target proteins through protein-specific peptide. Compared to traditional methods like Western blot and ELISA, our LC/MS-based protein quantification service offers several advantages. Our biomarker quantification bypasses the need for antibodies while maintaining exceptional specificity. Moreover, it boasts high throughput, especially when combined with MRM/PRM techniques, allowing for simultaneous quantification of multiple target proteins in a single experiment. Additionally, our technique provides a wide quantification range, accommodating both low-abundance and high-abundance proteins effectively.

Biomarker Quantification by LC/MS Project Workflow

Biomarker Quantification by LC/MS Application Display

Protac mediated degradation of target proteins

Protac usually degrades target proteins efficiently, and target concentrations are usually monitored by immunoaffinity methods due to the high sensitivity and low target concentration after degradation. However, this method is not always effective, in some cases, the binding between the antibody and the target may be interfered by other proteins, resulting in the inability to accurately quantify the target protein. In this case, LCMS/MS or immunoaffinity enrichment coupled with LCMS/MS can be considered for accurate quantification of the target protein.

From Khan, S., et al. A selective BCL-XL PROTAC degrader achieves safe and potent antitumor activity. Nat Med 25, 1938–1947 (2019).