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Data-independent acquisition (DIA) is a holographic mode of mass spectrometry data acquisition based on electrostatic field orbitrap. Compared to data-dependent acquisition (DDA), DIA has the advantages of panoramic scanning, deeper data coverage, high data reproducibility and accurate quantification. DIA allows independent analysis of each clinical sample within large-cohort research, and it can retain the integrity of sample information for essential data analysis.

DIA Proteomics Workflow


DIA Proteomics Service Project Workflow

DIA Proteomics Sample Requirements

Sample type

Sample

Recommended Volume

Body fluid

Plasma. serum

0.05~0.2 mL

Urine

50~100 mL

Cerebrospinal fluid, synovial fluid, ascites……

0.05~5 mL

Animal or human milk

2~50 mL

Animal tissue

Brain, heart, liver, spleen, lungs, kidneys, muscles, skin, or other tissues

15 mg~2 g

Plant tissue

Soft tissues of woody and herbaceous plants

50 mg~2 g

Cells

Cells

0.5×107~1×107

Special sample

FFPE

5-10 µm thick, 50 mm2 size, ≥10 slices

Biopsy or puncture tissue

1-3 needles, visible to the naked eye, the size of millet grains

* Please ensure proper pre-processing and storage methods for the samples.

* Please contact info@inomixo.com if you have any questions regarding the samples.


DIA Proteomics Analysis Content Display

Protein and Peptides Identification Column Chart
Differential Protein Expression Volcano Plot
Protein-Protein Interaction Module Analysis

INOMIXO can provide a variety of bioinformatics and customized analysis. For more proteomics analysis needs, please contact info@inomixo.com.

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Get In Touch With INOMIXO
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