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Unlike DDA methods that acquire MS/MS (or MS2) data for selected precursor ions, the DIA technique overcomes the inherent non-reproducibility and undersampling limitations of DDA by acquiring MS1 and MS2 data simultaneously without biasing precursor ion selection. The DIA technique can acquire MS2 spectra of all precursor ions within an isolation window to obtain a complete range of full mass range MS /MS data without the need for prior knowledge of the precursors, which is required for other target methods, including selective reaction monitoring and parallel reaction monitoring. While still maintaining comparable characteristics of target data analysis in terms of accuracy, reproducibility and consistency.
Recent advances in data-independent acquisition mass spectrometry provide higher sensitivity and protein coverage than traditional data-dependent acquisition. DIA cycles through a set of pre-defined peptide precursor isolation windows, stepping through 400 - 1200 m/z across the entire liquid chromatography gradient. All peptides were simultaneously fragmented against the windows within an isolation zone and detected by tandem mass spectrometry. Peptides were identified by matching ion peaks in the mass spectrum to a spectral library containing information on the ion patterns of the peptide fragments and their chromatographic elution times. Data-dependent acquisition is currently the most commonly used comprehensive quantitative proteomics method, but in recent years, independent data acquisition has become increasingly popular due to its improved detection and quantification capabilities.
In scientific research, the stability and reliability of data is the basis for trusting the results of experiments, and DIA technology effectively avoids the data bias caused by ion competition and peptide inhibition in DDA technology by detecting all peptide ions at the same time, etc. The DIA proteomics service of INOMIXO adopts stringent quality control processes and optimized experimental design to ensure data stability and reliability, providing researchers with the most comprehensive proteomics services. Strict quality control process and optimised experimental design are adopted to ensure the stability and reliability of the data, and to provide researchers with authentic and credible experimental results.
With its unique advantages, DIA proteomics service shows a wide range of application prospects in biomedicine, agricultural science, environmental science and other fields. In the field of biomedicine, it can help researchers explore the pathogenesis of diseases and the mechanism of drug action; in the field of agricultural science, it can provide strong support for crop improvement and pest control; in the field of environmental science, it can provide important data support for environmental pollution monitoring and ecological protection. DIA proteomics service under the brand of INOMIXO, with its excellent performance and wide application prospects, is gradually becoming the first choice of scientific researchers.
In conclusion, DIA proteomics service is gradually surpassing DDA technology and becoming a new star in the field of proteomics with its innovative advantages such as comprehensive coverage, high throughput, stable and reliable data, accurate quantification and wide application prospects, etc. With its leading DIA proteomics service, INOMIXO provides unprecedented convenience and breakthroughs for the majority of scientific researchers, and will help the field of scientific research move forward to a brighter future. towards a more brilliant future.
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